Канал: Siddharth Srivastava

Adjust reading frame, Subclone BKK56 gene in expression vector pGEX5X3

Adjust reading frame, Subclone BKK56 gene in expression vector pGEX5X3

Construct pUC19 containing Green Fluorescent Protein GFP gene

Construct pUC19 containing Green Fluorescent Protein GFP gene

Directional cloning with compatible cohesive ends

Directional cloning with compatible cohesive ends

Directional cloning, Sub clone human erythropoietin gene from plasmid pTDL14 into expression vector

Directional cloning, Sub clone human erythropoietin gene from plasmid pTDL14 into expression vector

Directional cloning, Sub clone human erythropoietin gene from plasmid pTDL14 into expression vector

Directional cloning, Sub clone human erythropoietin gene from plasmid pTDL14 into expression vector

Directional cloning, using reverse complement function

Directional cloning, using reverse complement function

Express human erythropoietin gene as fusion protein

Express human erythropoietin gene as fusion protein

PCR based cloning, Clone Gene bbk56 from pTDL11 into expression vector pGEX3X

PCR based cloning, Clone Gene bbk56 from pTDL11 into expression vector pGEX3X

PCR based cloning, Clone GeneY from pTDL11 into cloning plasmid pUC19

PCR based cloning, Clone GeneY from pTDL11 into cloning plasmid pUC19

How to check your primers will work or not before ordering:experiment

How to check your primers will work or not before ordering:experiment

PCR based cloning

PCR based cloning

PCR Master Mix

PCR Master Mix

Assemble PCR assay

Assemble PCR assay

Assemble restriction digestion assay

Assemble restriction digestion assay

Measure DNA by spectrophotometer

Measure DNA by spectrophotometer

Make Agarose gel for electrophoresis

Make Agarose gel for electrophoresis

Dilute and make TE buffer

Dilute and make TE buffer

Import DNA into Serial Cloner software

Import DNA into Serial Cloner software

Download Serial Cloner 2 6

Download Serial Cloner 2 6

Make 70% EtOH to wash DNA

Make 70% EtOH to wash DNA

Subcloning

Subcloning

Dilution concept

Dilution concept

Download Serial Cloner 2 6

Download Serial Cloner 2 6

Import pNG10A from NCBI

Import pNG10A from NCBI

Subcloning, The DNA Lab

Subcloning, The DNA Lab

Introduction to Cloning Software, The DNA Lab

Introduction to Cloning Software, The DNA Lab

Assemble PCR Assay, The DNA Lab

Assemble PCR Assay, The DNA Lab

Secret DNA Message, The DNA Lab

Secret DNA Message, The DNA Lab

Restriction Digest, The DNA Lab

Restriction Digest, The DNA Lab

Import DNA Sequence into Cloning Program, The DNA Lab

Import DNA Sequence into Cloning Program, The DNA Lab

The DNA Lab, Molecular Cloning Strategy workshop

The DNA Lab, Molecular Cloning Strategy workshop

The DNA Lab workshop flavors

The DNA Lab workshop flavors