Yeast harvesting from a starter the Brulosophy overbuild method. Harvest and reuse your yeast from a starter and don't waste your time with washing your yeast from your frementer. Save money making a yeast starter for your homebrew.
I overbuild my yeast starter and harvest enough to ferment 6 gallons / 23 liters of wort and save enough for a starter so that I can propagate and overbuild this yeast strain again, generation after generation. You can keep this process going and you can easily get over 8 generations.

Yeast Starter Recipe:
1 gram DME for every 10ml of water will give you an original gravity of 1.040
100g DME + 1000ml water = 1.040 OG
200g DME + 2000ml water = 1.040 OG

If the OG (original gravity) is too far above 1.040 it creates too much of a good thing for the yeast. When the sugar and nutrient content is too concentrated it stresses the yeast out, you don't get their full potential and there is a chance for off flavors.

If the OG is to low there is not enough sugar and nutrient for the yeast to eat. So keep it right around 1.040 OG. In this case bigger is not better.


Yeast Starter Calculator:
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Water:
Use the same water you use for your brewing water. Use something without chlorine or chloramine.

Equipment:
Erlenmeyer Flask - [ Ссылка ] (2000ml is the smallest size my overbuild method works with) Bigger is better.
Stir Bar - [ Ссылка ]
Stir Plate - [ Ссылка ]
Scale - [ Ссылка ]
Yeast - Brewers Choice
Aluminum foil - [ Ссылка ]
DME (dried malt extract): [ Ссылка ] - Pilsen Malt or the lightest you can find.
Large Pot: I use a 6 quart stainless steel pot - [ Ссылка ]
Mason Jars: I am using the 1 quart size - [ Ссылка ]
Hot pad or ove glove - [ Ссылка ]
Digital infrared thermometer - [ Ссылка ]
2 Stage Water Filter - [ Ссылка ]
Cool Spoon - [ Ссылка ]

Stir Plate:
You can make your own DIY stir plate or buy one.
The parts to build a stir plate can add up quickly. It depends on how much stuff you have laying around or what you can find for free. I made my stir plate it took time and money if I had to do it again I would probably just buy one from Stir Starters.
I'm not too sure about the quality and longevity of some of the stir plates on amazon.
If you don't want to make your own stir plate check out the ones from Stir Starters. He is a home brewer, they have a lifetime warranty, and they are under $50.
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Steps:
2100ml of water without chlorine or chloramine.
Leave around 800ml of water in flask pour the rest into a large pot.
Add stir bar to erlenmeyer flask before you start heating it up this will prevent superheating.
Add your foil lid before things get hot.
Add DME (dried malt extract) to pot before the boil starts.
Once boil starts turn down heat and simmer flask and dme for 10 minutes.
Cool to room temperature before pitching your yeast.
Your yeast and wort should both be at room temperature within 2°f of each other.
Let it spin for 24-48 hours.
Sanitize your jar that will hold your starter that you are saving.
With everything in solution pour off 700-800ml to save as your future starter.

Here is where the different methods come into play.
For either method you choose, save 700-800ml of the starter mixture you just made in a sanitized jar for your future starter. The remaining starter will be pitched into your wort.
Method A: Save 700-800ml of the starter mixture into a sanitized jar for your future starter. Pour the remainder of your starter mixture into clean sanitized jars with lids, or you can keep the remainder in the flask. Put them in the fridge to chill for 24-36 hours until all the yeast has settled into cakes on the bottom of the jars. Decant (pour off) the supernatant (liquid on top of the yeast cakes). Be careful not to disturb the yeast as you pour off the liquid. Keep enough liquid so that you can swirl the yeast into a pourable slurry, around 200ml.
Method B: Save 700-800ml of the starter mixture into a sanitized jar for your future starter. Keep the yeast for your wort in the flask. You can pitch all of it into your fermenter: the starter beer and yeast together, the whole thing. No need to decant.



Brulosophy:
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Links Below:

Blank Brew Sheet PDF: [ Ссылка ]

Amazon Gear List: [ Ссылка ]

Instagram: [ Ссылка ]

Website: [ Ссылка ]

How long will it keep viable in the fridge? 6 months no problem
Viability should be similar to commercial yeast. If the starter has been in the fridge sitting under beer for more than 6+ months I will do a two step starter. The overbuild calculator will help you with this.

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