4D live imaging of pre-stimulated antigen presenting cells (APCs, namely dendritic cells and macrophages, obtained after isolation and in vitro differentiation of bone marrow cells from C57BL/6) cultured with freshly isolated “naïve T cells” from the spleen of OT-I mice and observed at a frequency of 1 image every 10 sec for 16 hours. Information on Z axis (depth) was processed so that a color scale (a gradient of color ranging from blue to pink) was applied to it, providing a sense of spacial organization in that axis. Full cells or cell components closer to the dish surface were colored blue, while pink accounted for cells or cellular content positioned further from the dish surface.
