Dynamic conformational changes of the Spike trimer structures filmed with 150 ms time resolution. The highly dynamic movement and flexibility of the Spike trimer captured in our high-speed AFM movies revealed a fast arc-like displacement of the three fully opened RBDs. The total range movement of all three RBD almost covered the full range of 360°, roughly divided into three 120° shares and with occasional small overlaps in the positions adopted by neighboring RBDs. Spike oriented sideways on mica – stalk (pink) and RBDs (red, green and blue) trajectories.
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