Next-Generation Sequencing is the modified and newer version of the Sanger Sequencing, it allows us to sequence larger piece of DNA. It is a highly complex technique, requires a lot of complicated machinery just to sequence a piece of DNA in a reaction. Fortunately Next-Generation Sequencing is lesser expensive than it was few year ago.
Next-generation sequencing, also called massively parallel sequencing, refers to methods in which millions of DNA templates are sequenced simultaneously in a single reaction. The general strategy begins with the fragmentation of cellular DNA into small pieces. In most techniques, the cellular DNA is then amplified by the polymerase chain reaction (PCR) and sequenced by a method that usually involves DNA polymerase. The nucleotide sequence data is then assembled, using computer algorithms, into a continuous genome sequence.
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