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Collection of Serum- and Feeder-free Mouse Embryonic Stem Cell-conditioned Medium for a Cell-free Approach - a 2 minute Preview of the Experimental Protocol
Yun-Ui Bae, Hoon-Ki Sung, Jae-Ryong Kim
Yeungnam University, Department of Biochemistry and Molecular Biology and Smart-aging Convergence Research Center, College of Medicine; The Hospital for Sick Children Research Institute, Physiology and Experimental Medicine Program; University of Toronto, Department of Laboratory Medicine and Pathobiology;
This protocol provides a method for the collection of mouse embryonic stem cell (mESC)-conditioned medium (mESC-CM) derived from serum (fetal bovine serum, FBS)- and feeder (mouse embryonic fibroblasts, MEFs)-free conditions for a cell-free approach. It may be applicable for the treatment of aging and aging-associated diseases.
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