In this video I have explained about Interpretation of HPLC reports for Hemoglobinopathies in India
This video will be useful for all medical laboratory professional to interprete HPLC chromatograph and diagnose Hemoglobinopathies like sickle trait, sickle disease,beta thalassemia, HbE trait and disease, Hb D Punjab ( Heterozygous and Homozygous) , Hb lepore, HbJ Meerut, Hb Q India, HPFH and Double Heterozygous conditions in India
It took me three years to learn all different hemoglobinopathies which is covered in just 22 minute video !
#hplcreportinterpretation
#hplc
#hemoglobinopathies
#hplcforhemoglobinopathies
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TIMELINE FOR HPLC CHROMATOGRAPH INTERPRETATION VIDEO :
00:00 Introduction to HPLC chromatograph interpretation
00:11 Brief principle of HPLC
00:52 Common Hemoglobinopathies in India
02:01 Alpha gene variants
02:30 Beta gene variants
03:52 Biorad variant windows
04:03 Strategies to be followed to diagnose Hemoglobinopathies
05:08 Things to look after in chromatograph
06:45 HbA2 distribution
(Based on concentration different Hemoglobinopathies identified)
07:48 Factors /Diseases affecting HbA2
09:11 Chromatograph of beta thalassemia trait
10:08 Chromatograph of sickle cell trait
10:37 Chromatograph of sickle cell disease
11:17 Chromatograph of HbE trait
11:43 Chromatograph of HbE disease
12:05 Chromatograph of HbD Heterozygous (HbD Punjab)
12:59 Chromatograph of HbD Iran
13:52 Chromatograph of HbQ India
14:30 Chromatograph of Hb Lepore
15:32 Chromatograph of HbJ Meerut
16:21 Chromatograph of HbC trait
16:42 Windows wise Hemoglobinopathies (worldwide)
17:41 Examples of double Heterozygous
conditions
19:19 HPFH homozygous / Beta thalassemia major
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Video Highlights
What is normal range of HPLC?
What is HPLC?
How thalassemia diagnosed in HPLC?
What does it mean if HbA2 is high?
What if HbA2 is low?
Hemoglobin pattern analysis
Thalessemia and Hemoglobinopathies testing by HPLC
Detection of abnormal hemoglobin variants by HPLC methods
