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Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells - a 2 minute Preview of the Experimental Protocol

Papri Chatterjee, Yuri Cheung, Chee Liew
University of California Riverside, UCR Stem Cell Center, Department of Cell Biology and Neuroscience;

Despite recent advancements in genetic modification, transfection of human embryonic stem cells (HESCs) remains a capricious process. To our knowledge, systematic and efficient methods to transfect human induced pluripotent stem cells (iPSCs) have not been reported. Here, we describe robust protocols to efficiently transfect and nucleofect human iPSCs.

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