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A High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion - a 2 minute Preview of the Experimental Protocol

Anthony M. Esposito, Alexandra Y. Soare, Foramben Patel, Namita Satija, Benjamin K. Chen, Talia H. Swartz
Icahn School of Medicine at Mount Sinai, Immunology Institute, Division of Infectious Diseases, Department of Medicine; New Jersey City University, Department of Biology;

We describe a cell-based assay to report on HIV-1 fusion via the expression of green fluorescent protein detectable by flow cytometry or fluorescence microscopy. It can be used to test inhibitors of viral entry (specifically at the fusion step) in cell-free and cell-to-cell infection systems.

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