Aligning the ICSI (Intracytoplasmic Sperm Injection) machine for IVF (In Vitro Fertilization) is a crucial step in the process of fertilizing an egg with a single sperm. The alignment process ensures accuracy and precision during the injection. Here is a step-by-step guide for embryologists to align the ICSI machine:

Preparation:
Ensure that the ICSI machine and workstation are clean and sterile.
Check the availability of all necessary tools and equipment.
Microscope Setup:
Adjust the height of the microscope to a comfortable level for the embryologist.
Turn on the microscope and adjust the light intensity for optimal visibility.
Micromanipulator Calibration:
Check that the micromanipulator is properly calibrated. This involves ensuring that the X, Y, and Z axes are correctly aligned.
Use a calibration slide or calibration software provided by the manufacturer.
Pipette Calibration:
Calibrate the injection pipette by setting the desired injection volume and ensuring accurate delivery.
Verify that the pipette movement is smooth and precise.
Temperature Control:
Confirm that the temperature of the ICSI workstation is stable and set to the optimal temperature for handling gametes and embryos.
Sperm Preparation:
Prepare the sperm sample for injection. Ensure that the sperm are of good quality and concentration.
Load the sperm into the injection pipette.
Egg Preparation:
Prepare the egg(s) for injection. Ensure that the eggs are properly denuded and positioned for easy access.
Alignment of Pipette and Egg:
Using the micromanipulator controls, position the injection pipette over the egg.
Optimize the focus and magnification for clear visualization of the egg and sperm.
Injection Process:
Stabilize the egg with a holding pipette.
Carefully position the injection pipette and insert it through the zona pellucida into the cytoplasm of the egg.
Deliver a single sperm into the egg cytoplasm.
Post-Injection Inspection:
After injection, inspect the egg to ensure that the sperm has been successfully injected.
Remove the injection pipette gently to avoid damage.
Incubation:
Place the injected eggs in a suitable incubator for further development.
Documentation:
Record the details of the injection process, including the sperm used, the time of injection, and any observations made during the procedure.
Quality Control:
Periodically perform quality control checks on the ICSI machine to ensure continued accuracy and precision.
Clean-Up:
After completing the procedure, clean and sterilize all equipment and the workstation for the next use.
Documentation and Reporting:
Document the outcomes of the ICSI procedure, including fertilization rates and any observed abnormalities.

свернуть