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Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay - a 2 minute Preview of the Experimental Protocol
Heying Cui, Kyle M. Loftus, Crystal R. Noell, Sozanne R. Solmaz
State University of New York at Binghamton, Department of Chemistry;
Cyclin-dependent kinase 1 (Cdk1) is activated in the G2 phase of the cell cycle and regulates many cellular pathways. Here, we present a protocol for an in vitro kinase assay with Cdk1, which allows the identification of Cdk1-specific phosphorylation sites for establishing cellular targets of this important kinase.
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