This tutorial covers normalization, dispersion estimation, statistical testing, filtering and multiple testing correction. The materials are available at
[ Ссылка ]
We have added some clarifications on (log) fold change and other statistical testing related terms here: [ Ссылка ]
04:02 Normalization
08:02 FPKM method and why you should use raw values
12:20 Dispersion estimation
17:33 Statistical testing (generalized linear models, edgeR, DESeq2)
21:30 Multiple testing correction
23:20 Filtering
24:43 DESeq2 result table
