The iQue® advanced flow cytometry platform and integrated Forecyt® software, provide an integrated solution, that rapidly profiles antibody internalization and other critical antibody characteristics. This data analysis and visualization with plate-level analytics, is used to accelerate antibody discovery, development and screening of antibody drug candidates, for potential drug efficacy and toxicity.
Watch this video narration of a poster, which describes a characterization of antibody internalization, viability and specificity: [ Ссылка ]
0:08 I’m Caroline Weldon one of the scientists in the assay development group at Sartorius
0:15 I’ll be walking you through one of our posters presented this year at Saito. This poster is entitled A High Throughput Multiplex Antibody Internalization Assay
0:26 shown here is the multiplex antibody internalization assay workflow. The assay consists of three components each 10 microliters labeled test antibody, cells encoded or not, and cell membrane integrity die
0:43 each component is prepared at 3x before addition for a final concentration of 1x and an assay volume of 30 microliters
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