Watch the Full Video at [ Ссылка ].
Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells - a 2 minute Preview of the Experimental Protocol
Kyle M. Loh, Amrita Palaria, Lay Teng Ang
Stanford University School of Medicine, Institute for Stem Cell Biology & Regenerative Medicine, Stanford-UC Berkeley Siebel Stem Cell Institute; Stanford University School of Medicine, Department of Developmental Biology;
This protocol details a monolayer, serum-free method to efficiently generate hepatocyte-like cells from human pluripotent stem cells (hPSCs) in 18 days. This entails six steps as hPSCs sequentially differentiate into intermediate cell-types such as the primitive streak, definitive endoderm, posterior foregut and liver bud progenitors before forming hepatocyte-like cells.
Visit [ Ссылка ] to explore our entire library of 14,000+ videos of laboratory methods and science concepts.
JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching.
Subscribe to our channel: [ Ссылка ]
