Haemoglobin Test Procedure | Hgb Blood Test Method | Blood Hemoglobin
here are the critical points explained briefly on measuring hemoglobin concentration using a biochemistry analyzer.
Your doctor may suggest a hemoglobin test if you're experiencing weakness, fatigue, shortness of breath or dizziness. These signs and symptoms may point to anemia or polycythemia vera. A hemoglobin test may help diagnose these or other medical conditions.
Step 1: Collecting the Blood Sample
Properly collect a patient's blood sample into an EDTA tube to prevent clotting.
Step 2: Preparing the Khan Tubes
Accurately measure and prepare two Khan tubes, one with 2ml and the other with 500ul of Drabskin reagent using micropipettes.
Step 3: Mixing the Blood Sample
Use a micropipette to add 10 ul of the patient's blood sample to the Khan tube containing 2ml of Drabskin reagent and let it sit for 3 minutes.
Step 4: Setting up the Biochemistry Analyzer
Select the Hemoglobin program on the biochemistry analyzer.
Zero the analyzer using distilled water and micropipettes for precision.
Step 5: Aspirating the Reagent Blank
aspirate the reagent blank from the 500ul Khan tube for calibration.
Step 6: Aspirating the Patient's Sample
Aspirate the Khan tube containing the patient's blood sample mixed with reagent using a micropipette.
Step 7: Observing the Result
Interpret the results displayed on the biochemistry analyzer, noting reference ranges and clinical significance.
