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Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution - a 2 minute Preview of the Experimental Protocol
Leighton H. Duncan, Mark W. Moyle, Lin Shao, Titas Sengupta, Richard Ikegami, Abhishek Kumar, Min Guo, Ryan Christensen, Anthony Santella, Zhirong Bao, Hari Shroff, William Mohler, Daniel A. Colón-Ramos
Yale University School of Medicine, Department of Neuroscience and Department of Cell Biology; Sloan Kettering Institute, Developmental Biology Program; University of Connecticut Health Center, Department of Genetics and Genome Sciences and Center for Cell Analysis and Modeling; National Institutes of Health, Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering; WormGUIDES.org,; Universidad de Puerto Rico, Instituto de Neurobiología, Recinto de Ciencias Médicas;
Here, we present a combinatorial approach using high-resolution microscopy, computational tools, and single-cell labeling in living C. elegans embryos to understand single cell dynamics during neurodevelopment.
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