Welcome to Science With Tal!
In this clip of the Signal Propagation in the Neuron video, we will go over the voltage clamp apparatus and the different results we can get from it to better understand the underlying mechanisms of the action potential. You will see here and in the comment section the time stamps associated with each topic that I’ve covered.
Note that the graphs are not up to scale but the general shapes of the curves reflect the true behaviours of the variables in question.
To improve the quality of my content, I highly value the feedback from the viewer so do not hesitate to give any feedback in the comment section.
Timestamps
0:00 Introduction
0:22 Historical perspective on the action potential recordings
1:13 Voltage clamp apparatus explained
3:15 What happens with the capacitive current
4:20 Trial 1: Small hyperpolarization (Vc = -70 mV)
5:23 Trial 2: Small depolarization (Vc = -50 mV)
5:42 Voltage clamp convention (inward/outward)
7:10 Trial 3: Big depolarization (Vc = 0 mV)
8:10 Voltage clamp experiment at different Vc values
8:50 Voltage clamp with no external Na
9:38 Voltage clamp with tetrodotoxin
10:07 Voltage clamp with tetraethylammonium
10:29 Final word on the ions responsible for the action potential
10:40 Current and conductance (K & Na) measurements from voltage clamp recordings
13:34 Similarities and differences of K & Na currents and conductances
14:55 Conclusion & references
Resources used
Here is a list of the resources that I’ve used to produce this video. (Author: title resource)
Dale Purves: Neuroscience (6th edition)
Eric Kandel: Principles of neural science (6th edition)
Alan Hodgkin & Andrew Huxley: A quantitative description of membrane current and its application to conduction and excitation in nerve
Alan Hodgkin & William Albert Hugh Rushton: The electrical constants of a crustacean nerve fibre.
To have more information on these resources, you can refer to the conclusion section where a more formal citation is provided.
Video credits
Writing: Tal Klimenko
Voice: Tal Klimenko
Animations: Tal Klimenko
Drawings: Tal Klimenko
Editing: Tal Klimenko
Introductory jingle: Thierry Du Sablond
Conclusion music: lukrembo - jay ([ Ссылка ])
