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Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions - a 2 minute Preview of the Experimental Protocol
Melinda L. Koyle, Madeline Veloz, Alec M. Judd, Adam C.-N. Wong, Peter D. Newell, Angela E. Douglas, John M. Chaston
Brigham Young University, Department of Plant and Wildlife Sciences; Cornell University, Department of Entomology; Cornell University, Department of Molecular Biology and Genetics; Harvard Medical School, Division of Infectious Diseases, Boston Children's Hospital; SUNY Oswego, Biological Sciences;
A method for rearing Drosophila melanogaster under axenic and gnotobiotic conditions is presented. Fly embryos are dechorionated in sodium hypochlorite, transferred aseptically to sterile diet, and reared in closed containers. Inoculating diet and embryos with bacteria leads to gnotobiotic associations, and bacterial presence is confirmed by plating whole-body Drosophila homogenates.
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