Presented By:
Alex Rigort
Michael Grange
Speaker Biography:
Alex Rigort works as a product marketing manager for Thermo Fisher Scientific. He developed and used cryo-focused ion beam instrumentation for applications in electron tomography at the Max Planck Institute of Biochemistry in Munich and has more than 15 years experience in cryo-electron microscopy.
Michael completed his D.Phil in structural biology at the University of Oxford, UK, where he applied FIB milling, electron cryo-tomography (cryo-ET) and super-resolution microscopy to investigate the trafficking and egress of viral progeny within cells. After his doctoral studies, he moved to the Max Planck Institute for Molecular Physiology, Germany, as an EMBO Long-Term Fellow. During his fellowship, he established cryo-ET as a core method, was involved in establishing high-throughput FIB-milling workflows, and combined them to investigate the structure and architecture of isolated mammalian muscle and human stem-cell-derived cardiomyocytes. In 2021, he joined the Franklin as a group leader where his research uses in-situ structural techniques to determine the impact of disease-relevant genetic alterations on the structural cell biology of axonal plasticity, with an emphasis on cytoskeletal trafficking and structural alterations within the neuron. He also develops methods for cryo-ET and the correlative imaging of larger tissues for structural studies.
Webinar:
Arctis Cryo-Plasma-FIB for throughput and connectivity in cellular cryo-electron tomography
Webinar Abstract:
Cellular cryo-electron tomography is a high-resolution technique that enables imaging of the molecular machinery of a cell at close-to-native conditions. With the advent of dedicated cryo-FIB instruments, modern energy filters, fast direct electron detectors and advanced data acquisition, exciting new opportunities have opened for cryo-electron tomography to shed light on fundamental questions in cell and structural biology. In this webinar we will present our next generation Arctis Cryo-PFIB microscope pioneering the systematic production of lamellae from cellular samples for cryo-electron tomography. The new microscope features a state-of-the-art plasma ion source, an automatic sample loading system, integrated fluorescence microscopy for correlative microscopy and direct connectivity to the Cryo-TEM. This enables the production of cryo-lamellae with higher throughput and better productivity than ever before.
Earn PACE Credits:
1. Make sure you're a registered member of LabRoots ([ Ссылка ])
2. Watch the webinar on the LabRoots Website ([ Ссылка ])
3. Click Here to get your PACE credits (Expiration date – SEP 21, 2024): ([ Ссылка ])
LabRoots on Social:
Facebook: [ Ссылка ]
Twitter: [ Ссылка ]
LinkedIn: [ Ссылка ]
Instagram: [ Ссылка ]
Pinterest: [ Ссылка ]
SnapChat: labroots_inc
