The urease test is used to determine the ability of an organism to split urea, through the production of the enzyme urease.

Procedure of Urease Test

Streak the surface of a urea agar slant with a portion of a well-isolated colony or inoculate slant with 1 to 2 drops from an overnight brain-heart infusion broth culture.Leave the cap on loosely and incubate the tube at 35°-37°C in ambient air for 48 hours to 7 days.Examine for the development of a pink color for as long as 7 days.Positive Reaction: Development of an intense magenta to bright pink color in 15 min to 24 h.

Examples: Proteus spp, Cryptococcus spp, Corynebacterium spp, Helicobacter pylori, Yersinia spp, Brucella spp, etc.

Negative Reaction:  No color change.
Examples: Escherichia, Shigella, Salmonella, etc

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