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Preparation of Synaptoneurosomes from Mouse Cortex using a Discontinuous Percoll-Sucrose Density Gradient - a 2 minute Preview of the Experimental Protocol
Pamela R. Westmark, Cara J. Westmark, Athavi Jeevananthan, James S. Malter
University of Wisconsin, Department of Pathology and Laboratory Medicine, Waisman Center for Developmental Disabilities; University of Wisconsin, Department of Biochemistry, Waisman Center for Developmental Disabilities;
A method to prepare translationally active, intact synaptoneurosomes (SNs) from mouse brain cortex is described. The method uses a discontinuous Percoll-sucrose density gradient allowing for the quick preparation of active SNs.
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