CONSIDER SUBSCRIBING 🥰
☕Buy me a coffee? [ Ссылка ]
🔬Or Patreon if that's your jam: [ Ссылка ]
📢Twitter: [ Ссылка ]
💻Discord: [ Ссылка ]
I built a 3D printed, scanning laser confocal microscope so I could collect 3D surface topology and profilometry data! The microscope stage is based on an OpenFlexure "Delta" stage, and the confocal optics are constructed with a 3D printed body, some lenses, a 405nm laser, pinhole and photodiode. The microscope is controlled by a Raspberry Pi (which controls an arduino to move the steppers).
It's not beautiful, it takes forever to scan, and the images are of dubious quality... but it works!
OpenFlexure: [ Ссылка ]
0:00 Intro
1:56 Confocal vs Widefield Microscopy
3:25 OpenFlexure Motion Platform
4:01 Confocal optical breakdown
8:00 Delta motion stage
8:43 Photodiode amplifier
9:14 Confocal Pinhole demonstration
10:31 Camera vs Photodiode
11:31 Data processing considerations
14:05 Images and results!
18:22 Optimizations
21:37 Discord! Come hang out with us!
DIY Scanning Laser Microscope
Теги
confocalmicroscopescanning laser confocal microscopelaser scanning confocal microscopelaser microscopeprofilometrytopologysurface profile3d profilelaser scanningconfocal microscope3d printeddiy3d printed microscopeopenflexurediy microscopemake a microscopeconfocal microscopyconfocal laser scanning microscopyconfocal laser scanning microscopebreaking tapsconfocal laser microscope3d scanninglaser scanning microscope
