The germ tube test is a diagnostic tool in microbiology used to identify Candida albicans, a yeast species. It involves inducing the yeast to form a tube-like structure (germ tube) from a yeast cell. This test helps differentiate Candida albicans from other Candida species.
The germ tube test is a laboratory procedure used to identify Candida albicans. Here's a basic outline of the procedure:
1. **Materials:**
- Candida albicans culture
- Human serum
- Sterile distilled water
- Microscope slides
- Inoculating loop
2. **Preparation:**
- Obtain a pure culture of Candida albicans.
- Inoculate a loopful of the yeast onto a microscope slide.
3. **Adding Serum:**
- Add a small drop of human serum to the yeast on the slide. Mix it thoroughly with the inoculating loop.
4. **Incubation:**
- Incubate the slide at 37°C for 2-3 hours. This mimics conditions in the human body.
5. **Observation:**
- After incubation, examine the slide under a microscope using a 10
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