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Large-Scale Production of Cardiomyocytes from Human Pluripotent Stem Cells Using a Highly Reproducible Small Molecule-Based Differentiation Protocol - a 2 minute Preview of the Experimental Protocol
Hananeh Fonoudi, Hassan Ansari, Saeed Abbasalizadeh, Gillian M Blue, Nasser Aghdami, David S Winlaw, Richard P Harvey, Alexis Bosman, Hossein Baharvand
Royan Institute for Stem Cell Biology and Technology, ACECR, Department of Stem Cells and Developmental Biology, Cell Science Research Center; Victor Chang Cardiac Research Institute, Developmental and Stem Cell Biology Division; University of New South Wales, St. Vincent´s Clinical School, Faculty of Medicine; University of New South Wales, School of Biotechnology and Biomolecular Sciences; University of Science and Culture, Department of Developmental Biology; The Children´s Hospital at Westmead, Heart Centre for Children; University of Sydney, Sydney Medical School; University of Science and Culture, Tehran, Iran, Department of Developmental Biology;
Here, we present a robust, fast and scalable cardiomyocyte differentiation protocol for human pluripotent stem cells (hPSCs). Cardiomyocytes derived using this large-scale method can provide sufficient cell numbers for their effective use in human cardiovascular disease modeling, high-throughput drug screening, and potentially clinical applications.
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