This new microscope allows scientists to image thick, opaque samples for the first time. Shining patterns of light on the sample (close-up in upper left corner) generates an image with alternating light and dark stripes, which is blurred where the light bounces off the sample and changes direction. By taking multiple images of different phases of the light pattern, and combining them, a computer can filter out the effects of scattered light and generate an accurate image.
By combining this approach with imaging along different angles, EMBL scientists were able to obtain three-dimensional movies of the developing fruit fly embryo for the first time.
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