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Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation - a 2 minute Preview of the Experimental Protocol

Emmanuel Bikorimana, Danica Lapid, Hyewon Choi, Richard Dahl
Harper Cancer Research Institute,; Indiana University School of Medicine, Microbiology and Immunology; University of Notre Dame, Department of Biological Sciences;

Manipulating temporal gene expression in differentiating embryonic stem cells (ESCs) can be achieved using inducible gene systems. However, generation of these cell lines is costly and time consuming. This protocol achieves rapid expression of a transgene in differentiating ES-derived cells and subsequent analysis of downstream hematopoietic differentiation.

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