Can hand sanitizer with 70% ethanol kill bacteria? This short video shows real-time killing of E. coli with 70% ethanol. The first 6 seconds show live bacteria. Application of a few drops of ethanol instantly kills all bacteria indicated by the absence of movement.

At Danish Technological Institute (DTI), we help importers and producers of antimicrobial products document the efficiency of their products. In addition to the required antimicrobial activity testing according to ISO/EN standards, we also provide video clips like this to document the effect in a visible and more tangible way, offering end-users a chance to see with their own eyes how the product actually works.

Learn more about our services here:
Removal of unwanted microorganisms: [ Ссылка ] (English)
Fjernelse af uønskede mikroorganismer: [ Ссылка ] (Danish)

Bacteria: Escherichia coli.
Samples: Micropore filter biofilm prepared by a drop of overnight bacteria onto a 0.22 µm polycarbonate filter placed on nutrient agar plate. The plate was incubated at 37 °C for 2 hours before microscopy.
Microscopy: Spinning disk microscope.
Recording: Time lapse imaging was performed on an Olympus IX83 inverted microscope using bright-field illumination and a 100x 1.49 NA TIRF objective. The acquisitions were obtained with a Hamamatsu ORCA-Flash4.0 sCMOS camera, and the system was controlled by Olympus CellSens software. Image analysis was performed in Olympus CellSens and Fiji/Image.

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