This video explains about Quorum Sensing: The Cell Signaling Pathway in Bacteria for communication and control various physiological activities.
Timestamp: Quorum Sensing
00:11 Introduction to Quorum Sensing
02:03 Quorum Sensing Functions
02:36 Quorum Sensing in Gram-positive and Gram-negative Bacteria
03:02 Quorum Sensing Systems Gram-negative bacteria
03:33 Quorum Sensing Systems Gram-positive bacteria
03:58 Mechanism of action
06:07 LuxI/LuxR System: Bioluminescence
Quorum Sensing is a bacterial communication phenomenon that allows bacteria to communicate using secreted signal molecule to assess their population density.
This process enables population of bacteria to collectively regulate gene expression and regulate behavior of the population.
Gram-positive and Gram-negative bacteria use quorum sensing communication circuits to regulate various of physiological activities and these include:
• Symbiosis,
• Virulence,
• Competence,
• Conjugation,
• Antibiotic production,
• Motility,
• Sporulation, and
• Biofilm formation.
In general, Gram-negative bacteria use acylated homoserine lactones [AHL] as autoinducers and Gram-positive bacteria use processed oligo-peptides to communicate.
References:
Miller MB, Bassler BL. Quorum sensing in bacteria. Annu Rev Microbiol. 2001;55:165-99. doi: 10.1146/annurev.micro.55.1.165. PMID: 11544353.
Ball AS, Chaparian RR, van Kessel JC. Quorum Sensing Gene Regulation by LuxR/HapR Master Regulators in Vibrios. J Bacteriol. 2017;199(19):e00105-17. Published 2017 Sep 5. doi:10.1128/JB.00105-17
Marijke Frederix, J. Allan Downie, Chapter 2 - Quorum Sensing: Regulating the Regulators
Related Videos:
---------------------------------------------------------------------------------------------------------------
Plotting Bacterial Growth Curve: [ Ссылка ]
Colony Forming Units: [ Ссылка ]
Serial Dilution Methods: [ Ссылка ]
Copy Number Calculation for qPCR: [ Ссылка ]
How to analyze Primer Sequence or Oligonucleotide sequence designed for PCR / qPCR: [ Ссылка ]
How to generate qPCR standard curve in excel and calculate PCR efficiency: [ Ссылка ]
Real Time qPCR optimization, Calculating PCR Efficiency: [ Ссылка ]
Taqman Assay Vs SYBR Green Assay: [ Ссылка ]
Resolving poor PCR efficiency: [ Ссылка ]
Calculating molecular weight of Unknown protein [ Ссылка ]
Designing qPCR Primers
[ Ссылка ]
Oligonucleotide Preparation & Purification [ Ссылка ]
How to check Oligo Concentration
[ Ссылка ]
Ещё видео!