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High Throughput Sequential ELISA for Validation of Biomarkers of Acute Graft-Versus-Host Disease - a 2 minute Preview of the Experimental Protocol
Bryan Fiema, Andrew C. Harris, Aurelie Gomez, Praechompoo Pongtornpipat, Kelly Lamiman, Mark T. Vander Lugt, Sophie Paczesny
University of Michigan , Pediatric Blood and Marrow Transplant Program;
High throughput validation of multiple candidate biomarkers can be performed by sequential ELISA in order to minimize freeze/thaw cycles and use of precious plasma samples. Here, we demonstrate how to sequentially perform ELISAs for six different validated plasma biomarkers1-3 of graft-versus-host disease (GVHD)4 on the same plasma sample.
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