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A Microfluidic Platform for High-throughput Single-cell Isolation and Culture - a 2 minute Preview of the Experimental Protocol

Ching-Hui Lin, Hao-Chen Chang, Chia-Hsien Hsu
National Health Research Institutes, Taiwan, Institute of Biomedical Engineering and Nanomedicine; National Chung Hsing University, Tissue Engineering and Regenerative Medicine; National Tsing Hua University, Institute of NanoEngineering and MicroSystems;

Here, we present a protocol for isolating and culturing single cells with a microfluidic platform, which utilizes a new microwell design concept to allow for high-efficiency single cell isolation and long-term clonal culture.

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