How to Set up HPLC Calibration Curve - External Standard Calibration Method is demonstrated in this video. A sample of unknown concentration containing a mixture of compounds is analysed using the Shimadzu i-Series LC-2050C 3D, which includes a photodiode array (PDA) detector. We will also demonstrate how to accurately prepare a set of standards for external calibration in order to obtain a good calibration curve.
This video provides a step-by-step demonstration of how to operate the Shimadzu LabSolutions software (real time analysis and post run analysis) in order to obtain a calibration curve, starting from setting up a method file to run a real time analysis all the way to processing the data in the post-run LabSolutions analysis window.
Shimadzu i-Series LC-2050C 3D:
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Photodiode Array Detector:
[ Ссылка ]
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