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A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells - a 2 minute Preview of the Experimental Protocol

Paul D. Bryson, Chupei Zhang, Chi-Lin Lee, Pin Wang
University of Southern California, Los Angeles, Mork Family Department of Chemical Engineering and Materials Science, Viterbi School of Engineering;

Here, we use retroviral transduction and concatemeric transfection to create a cell line that can express the components of a lentiviral vector (LV) in the absence of tetracycline. This LV encodes GFP and is pseudotyped with a glycoprotein, SVGmu, which is specific for a receptor on dendritic cells.

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