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From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028 - a 2 minute Preview of the Experimental Protocol
Anja Greule, Songya Zhang, Thomas Paululat, Andreas Bechthold
Albert-Ludwigs-Universität Freiburg, Germany, Department of Pharmaceutical Biology and Biotechnology; Universität Siegen, Department of Chemistry and Biology;
Here we present a detailed protocol of (A) the identification of a natural product with antibiotic activity, (B) the purification of the compound, (C) the first model of its biosynthesis, (D) genome sequencing/-mining and the (E) verification of the biosynthetic gene cluster.
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