This week features "High-speed super-resolution structured illumination microscopy based on fiber optics" by Professor Thomas Huser
Professor of Physics, Department of Physics at the University of Bielefeld.
Super-resolved structured illumination microscopy (SR-SIM) is among the most flexible, fastest and least perturbing fluorescence microscopy techniques capable of surpassing the optical diffraction limit. Current custom-built instruments are easily able to deliver two-fold resolution enhancement at video-rate frame rates, but the cost of the instruments is still relatively high and the physical size of the instruments is still prohibitively large. Here, I will present our latest efforts towards realizing a new generation of compact, cost-efficient and high-speed SR-SIM instruments. I will take you on a tour from the use of spatial light modulators, digital micromirror devices towards our latest system, which is based entirely on fiber-optics. I will discuss how this allows us to operate SR-SIM at multiple color channels and fields of view exceeding 100 µm x 100 µm, and I will show applications of fixed and live cell biological data obtained with these systems.
As part of the 'Imaging ONEWORLD' series, the focus of these lectures is on microscopy and image analysis methods and how to apply these to your research. Almost all aspects of imaging such as sample preparation, labelling strategies, experimental workflows, ‘how-to’ image and analyse, as well as facilitating collaborations and inspiring new scientific ideas will be covered. Speakers will be available for questions and answers. The organisers, CRUK CI core facility staff, Gurdon Institute, MRC-LMB, MRC Cancer Unit and the ICR will be able to continue the discussion and provide advice on your imaging projects.
