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Selecting and Isolating Colonies of Human Induced Pluripotent Stem Cells Reprogrammed from Adult Fibroblasts - a 2 minute Preview of the Experimental Protocol
Urszula Polak, Calley Hirsch, Sherman Ku, Joel Gottesfeld, Sharon Y.R. Dent, Marek Napierala
University of Texas M.D. Anderson Cancer Center, Department of Molecular Carcinogenesis and Center for Cancer Epigenetics; Poznan University of Medical Sciences, Department of Cell Biology; The Scripps Research Institute, Department of Molecular Biology;
We present a protocol for efficient reprogramming of human somatic cells into human induced pluripotent stem cells (hiPSC) using retroviral vectors encoding Oct3/4, Sox2, Klf4 and c-myc (OSKM) and identification of correctly reprogrammed hiPSC by live staining with Tra-1-81 antibody.
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