Rendering a transparent background in Adobe Animate is easy when using an Alpha channel. You will first want to go to file export video /media. Click on the text box that says "ignore stage color". This allows you to render an alpha channel. Next, change the format from H.264 (or whatever your default preset is) and change it to Quicktime. Next, change your preset to GoPro Cineform12RGB with alpha channel or Apple ProRes 4444 with alpha channel. That will do it!

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