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Rapid Protocol for Preparation of Electrocompetent Escherichia coli and Vibrio cholerae - a 2 minute Preview of the Experimental Protocol
Miguel F. Gonzales, Teresa Brooks, Stefan U. Pukatzki, Daniele Provenzano
University of Texas Brownsville, Department of Biological Sciences; University of Alberta, Department of Medical Microbiology and Immunology; University of Texas Brownsville, Department of Biomedical Sciences;
Electroporation is a commonly employed method for introducing DNA into bacteria in a process known as transformation. Traditional protocols for the preparation of electrocompetent cells are time consuming and labor intensive. This article describes an alternate, rapid, and efficient method for the preparation of electrocompetent cells presently employed by some laboratories.
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