This video will demonstrate how to prepare and microinject in vitro synthesized EGFP mRNA and a translational-blocking morpholino oligo against pkd2, a gene associated with autosomal dominant polycystic kidney disease (ADPKD), into 1-cell stage zebrafish embryos. It will then analyze the success of the mRNA and morpholino microinjections by verifying GFP expression and phenotype analysis.
