Originally broadcast on 18-Apr-2017. Presented by Allison Mayle

In this webinar, you will learn how to maximize your genome editing efficiency using CRISPR/Cas9 and how to apply this technique in your research.

The main points in the webinar will include:
- How to design guide RNAs using online tools specific to the genome and application of interest.
- Tips and practical advice to assist you in choosing and optimizing a CRISPR/Cas9 delivery system.
- How you can create precise mutations using homology-directed repair, including template design and cleavage site

An Essential Guide to CRISPR/Cas9 Editing Efficiency
While CRISPR/Cas9 editing is utilized in a wide variety of cell types, editing efficiency continues to pose a challenge to researchers. Join Dr. Allison Mayle, as she shares best practices for increasing CRISPR/Cas9 editing efficiency. Viewers will discover online tools to aid in CRISPR/Cas9 design and delivery and tips for optimizing your CRISPR/Cas9 experiments.

In this webinar, Dr. Mayle will review the factors influencing genome editing, including target sequence selection and CRISPR delivery methods. A comparison of plasmid and viral vector delivery will be provided, as well as an introduction to DNA-free CRISPR/Cas9 ribonucleoprotein reagents. Additionally, Dr. Mayle will cover best practices for CRISPR knock-in mutagenesis via homology-directed repair (HDR) and applications available from new Cas9 protein variants.

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