Studying Cryptosporidium using 3D organoids requires delivery of the parasite to the lumen. For this, we microinjected Cryptosporidium Parvum oocysts (5 µm diameter) using an Eppendorf Transjector 5246 using TransferTip (ES) needle (15 µm inner diameter). Stem cell rich spheroids (seen here) are ideal to visualise microinjection but are not optimal for the culture of Cryptosporidum. In this video, two spheroids are microinjected (video on loop) to the tune of In The Hall Of The Mountain King by Grieg.

#Science #Microscopy #Microinjection

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