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Creating Adhesive and Soluble Gradients for Imaging Cell Migration with Fluorescence Microscopy - a 2 minute Preview of the Experimental Protocol
Siti Hawa Ngalim, Astrid Magenau, Ying Zhu, Lotte Tønnesen, Zoe Fairjones, J. Justin Gooding, Till Böcking, Katharina Gaus
The University of New South Wales, Centre for Vascular Research and Australian Centre for Nanomedicine; The University of New South Wales, School of Chemistry and Australian Centre for Nanomedicine;
A method for the assembly of adhesive and soluble gradients in a microscopy chamber for live cell migration studies is described. The engineered environment combines antifouling surfaces and adhesive tracks with solution gradients and therefore allows one to determine the relative importance of guidance cues.
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