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Affinity Labeling Detection of Endogenous Receptors from Zebrafish Embryos - a 2 minute Preview of the Experimental Protocol

Tonatiuh Molina-Villa, Valentín Mendoza, Fernando López-Casillas
Universidad Nacional Autónoma de México, Cell and Developmental Biology, Instituto de Fisiología Celular;

A novel technique for the detection of low abundance endogenous receptors present in zebrafish embryos is described. We have named it AFLIP because it consists of affinity labeling of the receptor by its ligand linked to immunoprecipitation.

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