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Identification of Skeletal Muscle Satellite Cells by Immunofluorescence with Pax7 and Laminin Antibodies - a 2 minute Preview of the Experimental Protocol
Xuesong Feng, Faiza Naz, Aster H. Juan, Stefania Dell'Orso, Vittorio Sartorelli
National Institutes of Health, Laboratory of Muscle Stem Cells and Gene Regulation; National Institutes of Health, Office of Science and Technology, National Institute of Arthritis, Musculoskeletal and Skin Diseases (NIAMS);
The precise identification of satellite cells is essential for studying their functions under various physiological and pathological conditions. This article presents a protocol to identify satellite cells on adult skeletal muscle sections by immunofluorescence-based staining.
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