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Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells - a 2 minute Preview of the Experimental Protocol

Carissa Dege, Christopher M. Sturgeon
Washington University in St. Louis, Department of Internal Medicine, Hematology Division;

Here, we present human pluripotent stem cell (hPSC) culture protocols, used to differentiate hPSCs into CD34+ hematopoietic progenitors. This method uses stage-specific manipulation of canonical WNT signaling to specify cells exclusively to either the definitive or primitive hematopoietic program.

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