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4D Microscopy of Yeast - a 2 minute Preview of the Experimental Protocol
Natalie Johnson, Benjamin S. Glick
University of Chicago, Department of Molecular Genetics and Cell Biology;
This protocol describes the analysis of fluorescently labeled intracellular compartments in budding yeast using multi-color 4D (time-lapse 3D) confocal microscopy. The imaging parameters are chosen to capture adequate signals while limiting photodamage. Custom ImageJ plugins allow labeled structures to be tracked and quantitatively analyzed.
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