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In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells - a 2 minute Preview of the Experimental Protocol

Jianle Wang, Montserrat C. Anguera
University of Pennsylvania, Department of Biomedical Sciences, School of Veterinary Medicine;

Here, we present a protocol to efficiently generate human trophoblastic cells from human pluripotent stem cells using bone morphogenic protein 4 and inhibitors of the Activin/Nodal pathways. This method is suitable for the efficient differentiation of human pluripotent stem cells and can generate large quantities of cells for genetic manipulation.

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