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Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays - a 2 minute Preview of the Experimental Protocol
Monica Frega, Sebastianus H. C. van Gestel, Katrin Linda, Jori van der Raadt, Jason Keller, Jon-Ruben Van Rhijn, Dirk Schubert, Cornelis A. Albers, Nael Nadif Kasri
Radboudumc, Department of Cognitive Neurosciences; Radboud University, Donders Institute for Brain, Cognition and Behaviour; Radboudumc, Department of Human Genetics; Radboud University, Department of Molecular Developmental Biology;
We modify and implement a previously published protocol describing the rapid, reproducible, and efficient differentiation of human induced Pluripotent Stem Cells (hiPSCs) into excitatory cortical neurons12. Specifically, our modification allows for control of neuronal cell density and use on micro-electrode arrays to measure electrophysiological properties at the network level.
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