Mastering PCR: 7 Tips for Accurate and Reliable Results| How to Avoid Common PCR Mistakes
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In molecular biology and biotech lab, the PCR, or polymerase chain reaction, is an effective instrument. Yet, even a small error can harm your outcomes. Here are seven suggestions to prevent PCR errors: Use only top-notch DNA samples, first. Second, keep each step's workstation and tools separate to prevent contamination. Next, maximise the concentrations of your primer and template. Running a positive and negative control with each PCR reaction is the fourth step. Lastly, confirm that your thermocycler is accurately calibrated. Sixth, refrain from utilising degraded or expired reagents. Finally, before beginning, double check your reagents and protocols. These recommendations will help you get trustworthy and accurate PCR findings.
