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Quantitative Assessment of Immune Cells in the Injured Spinal Cord Tissue by Flow Cytometry: a Novel Use for a Cell Purification Method - a 2 minute Preview of the Experimental Protocol
Hal X. Nguyen, Kevin D. Beck, Aileen J. Anderson
University of California, Institute for Memory Impairments and Neurological Disorders; University of California, Physical Medicine & Rehabilitation; University of California, Anatomy & Neurobiology; University of California, Sue and Bill Gross Stem Cell Research Center; University of California, Section of Molecular Biology; University of California, Reeve-Irvine Research Center;
Quantification of cellular inflammation in the injured/pathological CNS by flow cytometry is complicated by lipid/myelin debris that can have similar size and granulation to cells, decreasing sensitivity/accuracy. We have advanced a cell preparation method to remove myelin debris and improve cell detection by flow cytometry in the injured spinal cord.
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