How does the egg chamber obtain its oval shape? By imaging live egg chamgers, Haigo and Bilder recently found that germline nuclei (red) inside the chamber rotate in concert with the follicle layer surrounding them (green) during this elongation. They hypothesize that the spinning triggers the extracellular matrix to form a "girdle" around the egg, restraining the follicle layer's growth along the short axis.
4D live imaging of an ovariole expressing His2Av-mRFP and Indy-GFP with 87 µm maximal projection. Egg chambers rotate in stages 6 and 7 but not stages 4 and stage 9. The movie was acquired on a Zeiss LSM 5 Live (3 hours; 5 min time points).
Video courtesy of Saori Haigo and David Bilder, University of California, Berkeley. Reproduced from Haigo and Bilder (2011) ([ Ссылка ]). Copyright AAAS/all rights reserved.
See more reproduction images in the Cell Picture Show: [ Ссылка ]
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